<em>In vitro</em> Investigation of the MexAB Efflux Pump From <em>Pseudomonas aeruginosa</em>

In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa

There is an emerging scientific need for reliable tools for monitoring membrane protein transport. We present a methodology leading to the reconstitution of efflux pumps from the Gram-negative bacteria Pseudomonas aeruginosa in a biomimetic environment that allows for an accurate investigation of their activity of transport. Three prerequisites are fulfilled: compartmentation in a lipidic envir...

Investigation of MexAB-OprM efflux pump gene expression in clinical isolates of pseudomonas aeruginosa isolated from Intensive Care Unit

Background and Aims: Pseudomonas aeruginosa is one of the most important pathogens of nosocomial infections, especially in the ICU (Intensive Care Unit), which has resistance to a wide range of antibiotics, especially Carbapenems. Among the most important resistance mechanisms of this bacteria against carbapenems are MexAB-OprM efflux pump. Therefore, the aim of this study was to evaluate the g...

Characterization of the RND-type multidrug efflux pump MexAB-OprM of the plant pathogen Pseudomonas syringae.

In gram-negative bacteria, transporters belonging to the RND family are the transporters most relevant for resistance to antimicrobial compounds. In Pseudomonas aeruginosa, a clinically important pathogen, the RND-type pump MexAB-OprM has been recognized as one of the major multidrug efflux systems. Here, homologues of MexAB-OprM in the plant pathogens Pseudomonas syringae pv. phaseolicola 1448...

Investigation of an Efflux Pump Membrane Protein: A Roadmap

Effects of synthesized iron oxide nanoparticles from Ziziphora clinopodioides on expression of the efflux pump genes of Staphylococcus aureus

Introduction: One of the important factors causing nosocomial infections is drug-resistant Staphylococcus aureus strain, so correspondingly, having norA efflux pump is one of the bacterial's ability to generate drug resistance. Iron has been used as a nanoparticle with antimicrobial activity against bacteria. The aim of this study was to investigate the effect of iron oxide nanoparticles synthe...